Increasing thermal tolerance by somatic hybridisation of microalgae
Climate warming has interfered with many natural and biological processes leading to evidently adverse effects to crucial processes that support aquatic life. Coral cover has declined globally over recent decades due to high sean water temperatures caused by climate change. The coral thermal tolerance depends to a large extent on a symbiosis with single-celled microalgae, which provide most of the corals nutrition via translocation of photosynthates. Recently, bio engineering strategies have aimed to enha climate warming is halted. Hybridisation can be a tool to enhance diversity and phenotypes.
Hybridisation is a process where two or more atomic orbitals originating from the same atom are mathematically combined to produce an entirely new orbitals different from the original components. In relation to genomics, hybridisation involves combining two and/or RNA molecules together to form a . The formed double stranded molecule is stronger and more resistant to any type of affliction. In this project, I intend to explore the possibilities to generate hybridsbetween different microalgal phenotypes and between , i.e.,intraspecific and interspecific hybridisation.
Intraspecific hybridisation will involve combininggenetically different genotypes of the same species with the aim of producing a stronger hybrid species that is resistant to affliction caused by climate change and tolerant to thermal effects. By successfully combining different microalgae genotypes of the same species, we will produce a stronger microalgae offspring that will be stronger enough to withstand the effecting of climate warming and highly tolerant to temperature changes. This increases thermal tolerance translocation of photosynthesis will be restored, sufficient coral nutrition produces and in turn, restoration of the coral cover that is crucial to aquatic ecosystem. Interspecific hybridisation will involve combining two species from the same genus. In this process, genetic materials between the two species will be exchanged, leading to a recombination and the generation of individuals that are stronger and resistant to affiliations, unlike the parents.
The project will focus on learning laboratorytechniques of microalgae culturing, generating protoplasts from microalgae cultures,somatic hybridisation and physiological characterisation of the target species. Generatingprotoplasts of the microalgae will also lead to follow up experiments regarding geneticengineering with to understand their cellularmechanisms. This Masters project has the potential to be continued into a PhD project thatincludes genome sequencing of the hybrid microalgae, further physiological testing andcharacterisation of the hybrid phenotypes.
The outcome of the project will be an identification of a solution to the declining coral life cover that is threating the stability of the aquatic ecosystem.Somatic hybridisation of microalgaewill produce a more stronger species with high tolerance to temperature changes allowing the coral cover to thrive and grow despite the afflictions of climate warming. The goal is to increase resistance and thermal tolerance and protect the aquatic ecosystem until climate warming is halted.
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